Isolation of glycopeptides from Ricinus communis lectins.
نویسندگان
چکیده
منابع مشابه
Lectins as membrane components of mitochondria from Ricinus communis.
1. Mitochondria were isolated from developing endosperm of Ricinus communis and were fractionated into outer membrane and inner membrane. The relative purity of the two membrane fractions was determined by marker enzymes. The fractions were also examined by negative-stain electron microscopy. 2. Membrane fractions were sequentially extracted in the following way. (a) Suspension in 0.5M-potassiu...
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The agglutinins present in the seeds of Abrus precatorius and Ricinus communis have been separated from the toxins, abrin and ricin, and extensively purified. The procedure involves ion exchange chromatography on DEAEand CMcellulose columns, affinity chromatography on Sepharose 4B columns, and sucrose gradient centrifugation. The purified agglutinins which possessed almost no toxic activity wer...
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of human erythrocyte ghosts were solubilized with 0.5yc Triton X-100 in 56 mM sodium borate, pH 8.0. This procedure solubilized 51% of the membrane protein, 81% of the sialic acid, 89% of the receptors for the Agaricus bisporus lectin and 70 to 75% of the wheat germ agglutinin and Ricinus communis lectin receptors. The solubilized glycoproteins were then separated by affinity chromatography on ...
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The effects of ribosome-inactivating proteins (RIPs) from Ricinus communis and from Viscum album on the water permeability, Pf, and the surface dielectric constant, epsilon, of model membranes were studied. Pf was calculated from microelectrode measurements of the ion concentration distribution in the immediate vicinity of a planar membrane, and epsilon was obtained from the fluorescence of dan...
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iranian castor bean lectin was isolated and purified by several column chromatography methods. then, the sugar and amino acid content of the purified lectin was identified. castor bean lectin has some anti tumor and anti leukemia action. thus it may be used commercially for medical and biochemical purposes.
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1988
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.52.1771